Near Infrared Spectroscopy

Near infrared spectroscopy (NIRS) can be used to measure changes in oxygenated and de-oxygenated haemoglobin using near-infrared light (Siesler, Ozaki, Kawata & Heise, 2008). NIRS involves delivering infrared light into the head via a NIRS source. Infrared light from the NIRS source is absorbed or scattered as it enters tissue inside the head, such as the brain. The concentration of de-oxygenated haemoglobin and oxygenated haemoglobin affects how the infrared light is absorbed. As a result, changes in the oxygenation of haemoglobin will affect how much infrared light is absorbed by the brain. The extent of scattering and absorption is measured by a NIRS detector, which is placed near the NIRS source. This provides a mean of non-invasively assessing motor and cognitive activities, which are conventionally assessed using functional magnetic resonance imaging (fMRI) (Hoshi, 2005).

NIRS Applications

NIRS can also assess changes in haemoglobin oxygenation whilst participant’s are moving their entire body, which cannot be achieved with fMRI. For example, recent evidence has shown that fNIRS can be used to ‘encourage’ subjects to recruit the anterior prefrontal cortex (aPFC) in a neurofeedback procedure whilst they completed a movement imagery task (Ota et al., 2020).  The use of NIRS as a means of neurofeedback improved performance in a movement rehabilitation task. Moreover, NIRS also enabled neuroimaging during a movement task, revealing M1 and S1 and premotor oxygenation changes (Ota et al., 2020). This is an exciting means of usIng NIRS as a neurofeedback technique to improve rehabilitation outcomes, which are measured by a participant’s ability to move.

Despite its high spatial resolution, the combination of functional magnetic resonance imaging with TMS is very challenging. For instance, TMS coils introduce artefact is MRI measurements (eg. Bungert et al., 2010; Weiskopf et al., 2009), not to mention the difficulty associated with constructing coils that withstand the strong magnetic field in the MR environment. NIRS is an alternative measure that can be used to measure changes in oxygenation and avoid many of the difficulties associated with combining TMS and fMRI. NIRS can also be used in populations that are difficult to assess with fMRI, such as children or can simply exist as a chapter alternative. NIRS relies on an optical signal, where the scattering and absorption of light emitted by the NIRS source is measured by a NIRS detector. As a result NIRS is capable of measuring changes in the concentration of oxygenated an de-oxygenated haemoglobin can be measured during the application of brain stimulation in brain sites underneath and proximal to the site of stimulation. Recent experiments have applied repetitive TMS at different frequencies and reveal that increases in oxygenated haemoglobin when TMS applied at 2Hz and 5 Hz but not at 1 Hz (Cao et al., 2013). Given the the extraction of artefact free signals during brain stimulation can be challenging (e.g. Rogasch et al., 2017; Dowsett & Herrmann, 2016; Kohli & Casson, 2019), NIRS is a promising means of assessing the consequences of non-invasive brain stimulation.

A number of effects of TMS have been revealed on NIRS measurements. Groiss et al. (2013) applied four pulses of TMS to M1 and measured the consequences of these pulses using NIRS above M1, the prefrontal cortex and the primary sensory cortex bilaterally. NIRS revealed a decrease in in oxygenated haemoglobin compared to sham within M1, immediately beneath the site of stimulation (Groiss et al., 2013). Decreases in oxygenated haemoglobin were also observed within premotor cortex, a site functionally and anatomically connected to M1 (Groiss et al., 2013). To conclude, NIRS is a promising approach that has already been demonstrated to be sensitive to TMS, which can be used in the future to reveal causal evidence for the involvement of cortical regions in neurophysiological processes.

  1. Motor Imagery Training With Neurofeedback From the Frontal Pole Facilitated Sensorimotor Cortical Activity and Improved Hand Dexterity. Ota, Y., Takamoto, K., Urakawa, S., Nishimaru, H., Matsumoto, J., Takamura, Y., ... & Nishijo, H. Frontiers in Neuroscience. January 2020
  2. Removal of Gross Artifacts of Transcranial Alternating Current Stimulation in Simultaneous EEG Monitoring. Siddharth Kohli, Alexander J Casson. Sensors. January 2019
  3. Analysing concurrent transcranial magnetic stimulation and electroencephalographic data: A review and introduction to the open-source TESA software. Rogasch, N. C., Sullivan, C., Thomson, R. H., Rose, N. S., Bailey, N. W., Fitzgerald, P. B., ... & Hernandez-Pavon, J. C.. NeuroImage. February 2017
  4. Transcranial Alternating Current Stimulation with Sawtooth Waves: Simultaneous Stimulation and EEG Recording. James Dowsett and Christoph S. Herrmann. Frontiers in Human Neuroscience. March 2016
  5. A near infra-red study of blood oxygenation changes resulting from high and low frequency repetitive transcranial magnetic stimulation. Ting T Cao, Richard H Thomson, Neil W Bailey, Nigel C Rogasch, Rebecca A Segrave, Jerome J Maller, Zafiris J Daskalakis, Paul B Fitzgerald. Brain Stimulation. November 2013
  6. Quadri-pulse stimulation induces stimulation frequency dependent cortical hemoglobin concentration changes within the ipsilateral motor cortical network. Stefan Jun Groiss, Hitoshi Mochizuki, Toshiaki Furubayashi, Shunsuke Kobayashi, Setsu Nakatani-Enomoto, Koichiro Nakamura, Yoshikazu Ugawa. Brain Stimulation. January 2013
  7. Reducing image artefacts in concurrent TMS/fMRI by passive shimming. Bungert, Andreas, Chambers, Christopher D., Phillips, Mark and Evans, Christopher John. NeuroImage. 2012
  8. Near-Infrared Spectroscopy: Principles, Instruments, Applications. Siesler, H. W., Ozaki, Y., Kawata, S., & Heise, H. M.. John Wiley & Sons. 2008
  9. Image artifacts in concurrent transcranial magnetic stimulation (TMS) and fMRI caused by leakage currents: modeling and compensation. Weiskopf, N., Josephs, O., Ruff, C. C., Blankenburg, F., Featherstone, E., Thomas, A., ... & Deichmann, R. Journal of Magnetic Resonance Imaging. May 2009

Associated Products

The following products from our catalogue are associated with this technique. To find out more about these supported devices, follow the links below or get in touch via email or phone.


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